Phytochemical Characterization and In Vivo Efficacy of a Novel Hyptis suaveolens Vaginal Gel

Abstract

The objective of the present investigation was to design, develop, and evaluate a novel vaginal gel formulation incorporating petroleum ether extract of Hyptis suaveolens, along with its phytochemical characterization and in vivo therapeutic assessment. The plant extract was obtained through Soxhlet extraction [1] and subjected to qualitative phytochemical screening [2,9,11], which revealed the presence of several bioactive compounds such as flavonoids, terpenoids, tannins, and steroids. Multiple gel formulations were prepared using different polymers, including Carbopol 934P, HPMC K100, Pemulen TR-1, Pemulen TR-2, Lutrol F127, and xanthan gum [12,14]. These formulations were evaluated for key physicochemical parameters such as pH, viscosity, spreadability, extrudability, and drug content uniformity [5,13]. The optimized formulation was further subjected to in vivo evaluation using an experimental animal model to assess antimicrobial and anti-inflammatory efficacy [20]. Statistical analysis was carried out using one-way ANOVA, demonstrating significant differences between treated and control groups (p < 0.05) [13]. The results indicated that the formulated gel exhibited excellent physicochemical properties and substantial therapeutic effectiveness. The study highlights the potential of Hyptis suaveolens as a natural and effective alternative in the management of vaginal infections.