Isolation and Analytical Characterization of Azadirachta Indica Seed Constituents Using Chromatography and Infrared Spectroscopy

Abstract

To research neem and assess the impact of neem leaf extract in the forms of aqueous, ethanolic and methanol. several neem parts have yielded the isolation of over 140 compounds. There have been description of the entire neem tree, including the bark, leaves, flowers, seeds and furits in particular, inflammation, infection, fever, skin condition and dental issues. A simple thin layer chromatography digital image-based analytical methods has been developed for the quantitation of the botanical pesticides, azadirachtin. The method was validated by analyzing azadirachtin, process pesticide formulations, using acidifie vannilin reagent as a post chromatographic derivatizing agent. The separated azadirachtin was clearly identified as green spot. The Rf value was found 60 be 0.55, which is similar to reference standard. A standard calibration plot was established using a reference standard. A separation process is a method that converts a mixture or solution of chemical substances into two or more distinct products. Chromatographic is the one of the best technique for separation. Paper chromatography is the simplest and faster technique. Paper chromatography as the name indicates it carried out on paper. A simple filter paper can be used for it. Different type of paper can also be used, it depending on solvent. It detection analyzed by visually. Fluorescence of substances using UV, enzymatic and microbiological methods. Neem(Azadirachta indica ) is an indian tree well known for its several pharmacological activities, including antimicrobial activity. More than 300 compositions have already been isolated & azadirachtin is its main active component.